Pixel multiplexing for high-speed multi-resolution fluorescence imaging

Abstract

We introduce a imaging modality that works by transiently masking image-subregions during a single exposure of a CCD frame. By offsetting subregion exposure time, temporal information is embedded within each stored frame, allowing simultaneous acquisition of a full high spatial resolution image and a high-speed image sequence without increasing bandwidth. The technique is demonstrated by imaging calcium transients in heart cells at 250 Hz with a 10 Hz megapixel camera.