Kinetics of binding and geometry of cells on molecular biochips

Abstract

We examine how the shape of cells and the geometry of experiment affect the reaction-diffusion kinetics at the binding between target and probe molecules on molecular biochips. In particular, we compare the binding kinetics for the probes immobilized on surface of the semispherical and flat circular cells, the limit of thin slab of analyte solution over probe cell as well as hemispherical gel pads and cells printed in gel slab over a substrate. It is shown that hemispherical geometry provides significantly faster binding kinetics and ensures more spatially homogeneous distribution of local (from a pixel) signals over a cell in the transient regime. The advantage of using thin slabs with small volume of analyte solution may be hampered by the much longer binding kinetics needing the auxiliary mixing devices. Our analysis proves that the shape of cells and the geometry of experiment should be included to the list of essential factors at biochip designing.