Asymmetric Rotational Stroke in Mouse Node Cilia during Left-Right Determination

Abstract

Clockwise rotational movement of isolated single cilia in mice embryo was investigated in vivo. The movement generates leftward fluid flow in the node cavity and plays an important role in left-right determination. The leftward unidirectional flow results from tilting of the rotational axis of the cilium to posterior side. Because of the no-slip boundary condition at the cell surface, the upper stroke away from the boundary generates leftward flow, and the lower stroke close to the boundary generates slower rightward flow. By combining computational fluid dynamics with experimental observations, we demonstrate that the leftward stroke can be more effective than expected for cases in which cilia tilting alone is considered with the no-slip condition under constant driving force. Our results suggest that the driving force is asymmetric and that it is determined by the tilting angle and open angle of the rotating cilia. Specifically, it is maximized in the leftward stroke when the cilia moves comparatively far from the boundary.