Mathematical Model of a pH-gradient Creation at Isoelectrofocusing. Part III: Numerical Solution of the Non-stationary Problem

Abstract

The mathematical model describing the non-stationary natural pH-gradient arising under the action of an electric field in an aqueous solution of ampholytes (amino acids) is constructed and investigated. The model is part of a more general model of the isoelectrofocusing (IEF) process. To numerical study of the model we use the finite elements method that allows to significantly reduce the computation time. We also examine the influence of the different effects (taking into account the water ions, the various forms of Ohm's law, the difference between isoelectric and isoionic points of the substances) on the process IEF.