A simple and rapid method for detecting living microorganisms in food using laser speckle decorrelation

Abstract

Measuring microorganisms in food products is a critical issue for food safety and human health. Although various approaches for detecting low-levels of microorganisms in food have been developed, they require high-cost, complex equipment, invasive procedures, and skilled technicians which limit their widespread use in the food industry. Here, we present a simple, non-destructive, non-contact, and rapid optical method for measuring living microorganisms in meat products using laser speckle decorrelation. By simply measuring dynamic speckle intensity patterns reflected from samples and analyzing the temporal correlation time, the presence of living microorganisms can be non-invasively detected with high sensitivity. We present proof-of-principle demonstrations for detecting E. coli and B. cereus in chicken breast tissues.