Mixed Effect Dirichlet-Tree Multinomial for Longitudinal Microbiome Data and Weight Prediction

Abstract

Quantifying the relation between gut microbiome and body weight can provide insights into personalized strategies for improving digestive health. In this paper, we present an algorithm that predicts weight fluctuations using gut microbiome in a healthy cohort of newborns from a previously published dataset. Microbial data has been known to present unique statistical challenges that defy most conventional models. We propose a mixed effect Dirichlet-tree multinomial (DTM) model to untangle these difficulties as well as incorporate covariate information and account for species relatedness. The DTM setup allows one to easily invoke empirical Bayes shrinkage on each node for enhanced inference of microbial proportions. Using these estimates, we subsequently apply random forest for weight prediction and obtain a microbiome-inferred weight metric. Our result demonstrates that microbiome-inferred weight is significantly associated with weight changes in the future and its non-trivial effect size makes it a viable candidate to forecast weight progression.