Shot noise limited high speed stimulated Raman microscopy
Abstract
We report a shot noise limited high speed stimulated Raman microscopy platform allowing to acquire molecular vibrational spectra over 200 in 12 at a scan rate of 40kHz. Using spectral focusing together with optimized acousto-optics programmable dispersive filters the designed low noise imaging platform performs chemical imaging of dynamical processes such as Mannitol crystal hydratation and reaches a signal to noise ratio sufficient to perform label free histological imaging on frozen human colon tissue slides.
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