Structured illumination microscopy based on fiber devices

Abstract

We present a simple and compact approach of structured illumination microscopy by using three $2\times2$ fiber couplers and one $1\times4$ MEMS optics switch. One uniform and three fringe illumination patterns were produced by placing seven output fiber tips at the conjugate Fourier plane of the illumination path. Stable and relatively high-speed illumination switching was achieved by the optics switch. Super-resolution and optical sectioned information was reconstructed from 4-frame data by using algorithms based on a joint Richardson-Lucy deconvolution method and a Hilbert transform method. By directly removing the out-focus components from the raw images provides better imaging results.