Binding of Carminomycin to synthetic polyribonucleotides poly(A) and poly(U): absorption and polarized fluorescence study

Abstract

Binding of anthracycline antibiotic carminomycin (CM) to synthetic polyribonucleotides poly(A) and poly(U) was studied in solution of low ionic strength in a wide phosphate-to-dye (P/D) range using absorption and polarized fluorescence spectroscopy. Two different modes of CM binding to the ss-polynucleotides have been identified. The first of them dominating at low phosphate-to-dye (P/D) ratios is self-assembly of the heterocyclic dye on the polymer surface driven by to cooperative electrostatic binding of amino group of CM sugar moiety to negatively charged polynucleotide phosphate groups with the chromophores self-stacking. At high P/D values, the stacking-associates disintegrate and monomeric binding of ligand to nucleic bases become prevalent. Thermodynamic parameters of binding were estimated for the both cases.