An analytic equation for single cell electrochemical impedance spectroscopy with a dependence on cell position

Abstract

An analytic equation for electrochemical impedance of a single-cell measured with a microelectrode is presented. A previously reported equation had a practical problem that it is valid only when the microelectrode resides at the center of the cell under test. In this work, we propose a new analytic equation incorporating dependence on cell position, and confirmed its effectiveness by numerical simulation. Comparisons show that our proposed equation gives an excellent agreement with simulated impedance values. Discrepancies between results from our equation and numerical simulation is suppressed within 13%, which is a dramatic reduction from the previously-reported equation as large as 58%. The proposed analytic equation is expected to enable more accurate analysis in actual cell experiments.