Estimation of In-Vitro Free Radical Scavenging and Cytotoxic Activities of Thiocarbohydrazones Derivatives

Abstract

Objective: To investigate free radical scavenging potential of thiocarbohydrazones derivatives for the discovery of antioxidant compounds of synthetic origin. Method: Eighteen thiocarbohydrazones derivatives were screened for antioxidant activities by using in vitro radical inhibition assay. Cytotoxicity all derivatives were evaluated using invitro cytotoxicity assay on mouse fibroblast cell line. Results: various compounds of the have shown significant radical scavenging activities as compared with the standard drug butylated hydroxytoluene. In addition in an in vitro cell cytotoxicity assay selected analogues were found to be non-cytotoxic against fibroblast 3T3 cell line. Conclusion: Taken together, these findings all compound this series could be further evaluated in in vivo model of oxidative stress to develop as a therapeutic agent against oxidative stress related disorders due to exhibiting excellent free radical scavenging properties and having their non cytotoxic nature against normal cells.