An Allosteric Model for the Influence of $\text{H}^+$ and $\text{CO}_2$ on Oxygen-Hemoglobin Binding

Abstract

In the physiology of oxygen-hemoglobin binding, an important role is played by the influence of $\text{H}^+$ and $\text{CO}_2$ on the affinity of hemoglobin for $\text{O}_2$. Here we extend the allosteric model of hemoglobin to include these effects. We assume purely allosteric modulation, i.e., that the modulatory effects of $\text{H}^+$ and $\text{CO}_2$ on oxygen binding occur only because of their influence on the T $\leftrightarrow$ R transition, in which all four subunits of the hemoglobin molecule participate simultaneously. We assume, moreover, that these modulatory influences occur only through the interaction of $\text{H}^+$ and $\text{CO}_2$ with the amino group at the N-terminal of each of the four polypeptide chains of the hemoglobin molecule. We fit the model to experimental data and obtain reasonable agreement with the observed shifts in oxygen-hemoglobin binding that occur when the concentrations of $\text{H}^+$ and $\text{CO}_2$ are changed.