Geometry-Dependent Defect Merging Induces Bifurcated Dynamics in Active Networks

Abstract

Cytoskeletal networks can repair defects to maintain structural integrity. However, the mechanisms and dynamics of defect merging remain poorly understood. Here we report a geometry-tunable merging mechanism in microtubule-motor networks initiated by active crosslinking. We directly generate defects using a light-controlled microtubule-motor system in O-shaped and V-shaped networks, and observe that the defects can self-close. Combining theory and experiment, we find that the V-shaped networks must overcome internal elastic resistance in order to zip up cracks, giving rise to a bifurcation of dynamics dependent on the initial opening angle of the crack: the crack merges below a critical angle and opens up at larger angles. Simulation of a continuum model reproduces the bifurcation dynamics, revealing the importance of overlapping boundary layers where free motors and microtubules can actively crosslink and thereby merge the defects. We also formulate a simple elastic-rod model that can qualitatively predict the critical angle, which is tunable by the network geometry.