Extended electrochemical monitoring of biomolecular binding using commercially available, reusable electrodes in microliter volumes

Abstract

Electrochemical biosensors ("E-AB" or "E-DNA" type sensors) that utilize square-wave voltammetry originated in academic labs with a few standard experimental configurations for the electrochemical cell and data analysis. We report here on adaptations of these approaches that are friendly to novice scientists such as those in undergraduate laboratories. These approaches utilize commercially available components, low volumes, work over extended periods and enable facile analysis using a custom excel sheet.