Miniaturized microscopes to study neural dynamics in freely-behaving animals

Abstract

Head-mounted miniaturized microscopes, commonly known as miniscopes, have undergone rapid development and seen widespread adoption over the past two decades, enabling the imaging of neural activity in freely-behaving animals such as rodents, songbirds, and non-human primates. These miniscopes facilitate numerous studies that are not feasible with head-fixed preparations. Recent advancements have enhanced their capabilities, allowing for faster imaging, larger fields of view, and deeper brain penetration. In this review, we examine the latest progress in one-photon and multi-photon miniscopes. We highlight the unique opportunities these devices present for neuroscience research, discuss the current technical challenges, and explore emerging technologies that promise to advance the development of miniscopes.