Mycoponically Integrated Network Device for Multimodal Sensing with Living Mycelial Networks

Abstract

Living mycelial filaments integrate chemical, optical, mechanical, thermal, and biological information via electrophysiological cellular trans-membrane potential. The challenge is to create a mycology interface that sustains metabolism, standardizes electrode geometry, and tolerates mechanical damage. Using mycoponics we overcome these factors that limited prior demonstrations to single modalities, and operational windows of days to weeks. We present MIND, an engineered biophysical interface integrating antimicrobial nutrient delivery (ceramic size exclusion) with non-invasive electrophysiology, in cylindrical (MINDTube) and planar (MINDPixel) form-factors. The platform sustains colonized \textit{Pleurotus ostreatus} mycelium beyond 11 months and distinguishes 14 stimulus classes from a single unmodified device. Steady-state intensity responses follow Hill-type calibration functions across five phylogenetically diverse fungi grown on the identical interface, making strain selection a tunable design parameter. Multichannel decoding from the standardized electrode geometry recovers stimulus duration, location, and trajectory. Continuous nutrition provided by mycoponics recovered complete electrophysiological function within 72 h after mechanical excision. MIND converts living mycelium networks into universal, self-repairing, biosensors.