Quantification of Captopril using Ultra High Performance Liquid Chromatography

Abstract

We develop an efficient, sensitive, accurate, cost effective, and greener liquid chromatography method for the determination of a quantification method concerning Captopril in pharmaceutical dosage forms by using ultra high performance liquid chromatography (UHPLC) equipment. Satisfactory resolution was able to be achieved using a Water Aquity UHPLC BEH C18 1.7 $\mu$m $\times$ 2.1 mm $\times$ 50 mm column and an isocratic mobile phase composition of Methanol, Milli-Q water and Trifluoroacetic acid in the ratio of 55:45:0.05 v/v/v respectively at a flow rate of 0.1mL/min and a wavelength detection of 220nm. The retention time for Captopril was found to be 1.744 minutes while for Captopril disulfide it was found to be 2.657 minutes. The method was validated based on the ICH and USP guidelines.