Unraveling Primary Energy Conversion Dynamics in Intact Cells of Gemmatimonas groenlandica

Abstract

Photosynthetic bacteria have evolved specialized light-harvesting structures to efficiently capture sunlight. Here, we investigate the ultrafast dynamics in intact cells of Gemmatimonas groenlandica, featuring unique double-ring LHh-LH1 photosynthetic complexes containing bacteriochlorophyll a. Two-dimensional electronic spectroscopy with lock-in detection allows us to directly observe primary energy-conversion processes, from excitation-energy transfer within the antenna system to the formation of a charge-separated state in the reaction center, taking place on 50 ps time scale. Through comparison with Rhodospirillum rubrum, which lacks the outer antenna ring, we use a combination of global analysis and nodal/center line slope analyses to resolve LHh-LH1 excitation energy transfer which occurs on a ~1-2 ps timescale in Gemmatimonas groenlandica.