Unzipping Kinetics of Double-Stranded DNA in a Nanopore
Alexis F. Sauer-Budge, Jacqueline A. Nyamwanda, David K. Lubensky, Daniel Branton
Abstract
We studied the unzipping kinetics of single molecules of double-stranded DNA by pulling one of their two strands through a narrow protein pore. PCR analysis yielded the first direct proof of DNA unzipping in such a system. The time to unzip each molecule was inferred from the ionic current signature of DNA traversal. The distribution of times to unzip under various experimental conditions fit a simple kinetic model. Using this model, we estimated the enthalpy barriers to unzipping and the effective charge of a nucleotide in the pore, which was considerably smaller than previously assumed.
Create a lesson
Related papers
Competing routes to spontaneous flow in confined active nematics
Rahil N. Valani, Vedad Dzanic, Sumesh P. Thampi et al.
Scaling and Condensation of Dry Active Matter Around Circular Obstacles
Felipe P. S. Júnior, F. Q. Potiguar, Jorge L. C. Domingos et al.
Active Hydrodynamics Couples Polymer Organization, Shape Fluctuations, and Motility in Deformable Droplets
Ritu Raj, P. B. Sunil Kumar
Inferring interactions between active particles using harmonic traps
Arnaud Compagnie, Joscha Mecke, Ivo Buttinoni et al.
Spontaneous filament formation and network self-assembly via active phase separation
Elena Lucas, Varun Venkatesh, Amin Doostmohammadi
Sensitivity of Nucleation Thermodynamics and Kinetics to the Treatment of Long-Range Interactions
Fernanda Sulantay Vargas, Kimia Sinaeian, Amir Haji-Akbari