Measurements of Protein-Protein Interactions by Size Exclusion Chromatography
J. Bloustine, V. Berejnov, S. Fraden
Abstract
A method is presented for determining second virial coefficients B2 of protein solutions from retention time measurements in size exclusion chromatography (SEC). We determine B2 by analyzing the concentration dependance of the chromatographic partition coefficient. We show the ability of this method to track the evolution of B2 from positive to negative values in lysozyme and bovine serum albumin solutions. Our SEC results agree quantitatively with data obtained by light scattering.
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