The noisy image retrieval for fluorescence digital microscopy

Abstract

The efficient optical tool for elimination of the phase and amplitude distortions produced by imperfectness of the optical elements in microscope lightpath considered. This robust procedure described by simple theoretical model proved to be successful to repair the noisy images with noise to signal ratio close to 100. It is shown that Van Cittert - Zernike theorem provides adequate description of imperfect microscope operating under both coherent and incoherent illumination conditions and having turbid media in object and intermediate planes.