Temperature Effects on Threshold Counterion Concentration to Induce Aggregation of fd Virus

Abstract

We seek to determine the mechanism of like-charge attraction by measuring the temperature dependence of critical divalent counterion concentration ($\rm{C_{c}}$) for the aggregation of fd viruses. We find that an increase in temperature causes $\rm{C_c}$ to decrease, primarily due to a decrease in the dielectric constant ($ε$) of the solvent. At a constant $ε$, $\rm{C_c}$ is found to increase as the temperature increases. The effects of $T$ and $ε$ on $\rm {C_{c}}$ can be combined to that of one parameter: Bjerrum length ($l_{B}$). $\rm{C_{c}}$ decreases exponentially as $l_{B}$ increases, suggesting that entropic effect of counterions plays an important role at the onset of bundle formation.